Common mistakes in cortisol lab testing often involve improper timing. Cortisol follows a strong circadian rhythm, so a single morning sample without a diurnal profile is rarely informative. Stress from the blood draw itself can elevate levels. Many consumer tests (e.g., saliva kits) lack standardization and clinical validation. Medications like corticosteroids or oral contraceptives interfere with results. Another frequent error is interpreting a single high value as disease – transient elevations due to poor sleep, psychological stress, or infection are common. Without repeat measurements and clinical correlation, no treatment should be started. Home tests may offer clues but cannot replace a doctor's evaluation. Always consider the full clinical picture before acting on cortisol results.
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